MITOCHONDRIAL LOCALIZATION OF OXIDATIVE ENZYMES: STAINING RESULTS WITH TWO TETRAZOLIUM SALTS
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چکیده
منابع مشابه
Mitochondrial Localization of Oxidative Enzymes: Staining Results with Two Tetrazolium Salts
A comparison is made of the staining results obtained with Nitro-BT and MTT-Co(++) as acceptors when DPNH is the substrate in frozen sections of cold formol-calcium-fixed rat kidney (normal and following ligation of the blood vessels) and human liver containing lipofuscin granules. The kidney results are evaluated in terms of mitochondrial morphology seen after classical mitochondrial stains an...
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ÖZ E T Bu çalışmada tetrazolyum tuzları ve formazanların yapıları tanımlanmıştır. Ayrıca formazan-tetrazolyum sistemlerinin önemi ve kullanım alanları açıklanmıştır. Formazanların sahip oldukları conjugate p-system kaynaklanan tautomerleşme özellikleri ve geometrik izomerleri incelenmiştir. Bu bileşiklerin en yaygın kullanılan sentezleri ve yapılarının açıklanmasında önemli olan UV-visible, IR ...
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Tetrazolium salts have proved useful as indicators of electron transfer. Compounds such as triphenyltetrazolium chloride' are more practical than indicators like methylene blue since they are converted from a soluble colorless salt to an insoluble formazan in the reduced state. They also provide similar convenience in detecting enzymatic activity and specific sites of such activity (Bielig, Kau...
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Methods are presented for the intramitochondrial localization of various diphosphopyridine nucleotide and triphosphopyridine nucleotide-linked dehydrogenases in tissue sections. The cytochemical reactions studied involve the oxidation of the substrates by a specific pyridino-protein. The electron transfer of tetrazolium salt is mediated by the diaphorase system associated with the dehydrogenase...
متن کاملCytochemical localization of certain oxidative enzymes.
Employing the technique of cell fractionation, Schneider and Hogeboom (’56) described the localization of certain oxidative enzymes. They reported that cytochrome oxidase and succinic dehydrogenase were found only in the mitochondrial fraction while isocitric dehydrogenase was present in the supernatant and DPN and TPN cytochrome c reductases were observed to be in both fractions. More recently...
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ژورنال
عنوان ژورنال: The Journal of Biophysical and Biochemical Cytology
سال: 1961
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.9.1.47